Technical Summary: Industrial
Glycerol
Analysis
(Bulletin ref. TB050147)
A simple,
rapid and direct
enzymatic analysis for glycerol in a wide variety of aqueous fluids
including beverage, pharmaceutical and fermentation extracts.
Name:
Code:
Size:
Instruments:
Glycerol
II Assay
GMRD-185
100ml
(140 analyzer
cycles)
GL6 and GM8 Series analyzers
Principle:
In
the presence of glycerol kinase (GK), glycerol is phosphorylated
by adenosine triphosphate (ATP) forming glycerol-3-phosphate
(G-3-P) which in turn is oxidized by glycerol-3-phosphate oxidase
(GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide:
i)
ii)
Under the conditions
of
the assay, reactions i) and ii) run concurrently in
the reaction
chamber and the rate of oxygen consumption is directly
proportional
to glycerol concentration.
Samples:
Sample
Volume:
Pre-dilution Ratio:
Beverages,
pharmaceuticals, fermentation extracts.
7 µl (variable
2 - 11 µl)
Typically 7:250 (sample: H2O)
Analytical
Range:
Analysis Time:
i) 0
- 4 %W/V, 0 - 40 g/L (typical pre-dilution)
ii) 0 - 100 mg% (direct
injection)
20 - 25 seconds
Analytical
Cycle Time:
Precision:
Analyzer Sensitivity:
Detection Limit:
Reagent Stability:
Approx.
60 seconds
S.D. = 0.01 at 2 %W/V
0.01 %; 0.1 mg%
Approx.
2 mg% (direct injection)
Shelf-life greater than 12 months unopened when stored at 0
- 5°C,
3- 4 weeks after reconstitution if stored at 0 - 5°C
between use.
