Technical Summary: HDL Cholesterol Analysis (Bulletin ref. TB059030E)
 

Analox High Density Lipoprotein (HDL) Reagent is intended for the quantitative determination of the HDL cholesterol fraction in plasma or serum and hence its relationship (%) to the total plasma or serum cholesterol.

The inverse relationship between HDL concentration and cardiovascular disease risk is well established. Epidemiological studies have stressed the association of low levels of HDL cholesterol with increased risk of ischemic heart and ischemic cerebrovascular disease. Thus measurement of the HDL cholesterol fraction is useful in identifying individuals at increased risk.
 

Name:
Codes:

HDL Cholesterol Microtubes
GMRD-089 (50 ready-to-use, pre-dosed reagent tubes)
 

Instruments:
 

All GM7/GL5 Series analyzers
 

Additional
Requirements:

Cholesterol Rate Reagent Kit (GMRD-084)
Suitable Microcentrifuge (e.g. Gilson GmCLab - see Note i) below)
 

Principle:

When plasma/serum is mixed with the lyophilized reagent, the dextran sulphate and magnesium ions present produce selective precipitation of non HDL lipoprotein fractions (LDL/VLDL/Chylomicrons). The HDL cholesterol fraction remains in the supernatant after high speed centrifugation. The supernatant HDL cholesterol concentration is measured and then compared to the plasma/serum total cholesterol.
 

Samples:
Sample volume:
Centrifuge Time:
 

Plasma or serum.
50 µl
2 minutes (Gilson GmCLab microcentrifuge)
 

Reagent Stability:

Shelf life 12 months unopened at 0 - 5°C.
 

Notes:

i) The Gilson GmCLab microcentrifuge is ideal for separation of both plasma and LDL/VLDL precipitation supernatant. Plasma separation can be achieved in approximately two minutes using Analox heparinized blood collection tubes, GMRD-067 (100/pk). The fill (0.5 ml) is appropriate for capillary site (finger prick) blood sampling.
ii) HDL cholesterol concentration is measured after transferring approximately
30 µl of supernatant to the individual esterase microtubes in the GMRD-084 reagent kit and proceeding as directed in the instructions.