Technical Summary: 3-Hydroxybutyrate Analysis (Bulletin ref. TB04866C)
 

A fast enzymatic analysis for plasma 3-Hydroxybutyrate.
 

Name:
Code:
Size:

3-Hydroxybutyrate
GMRD-130
20 tests; 140 analyzer cycles The number of tests may be doubled by using half-volumes (micro-method).
 

Instruments:
 

All GM7 Series analyzers
 

Principle:

i) The enzyme 3-hydroxybutyrate dehydogenase (3-HBDH) reduces NAD in proportion to the 3-hydroxybutyrate concentration in a brief pre-reaction:
 

ii) The rate of oxidation by peroxidase (POD) of the generated NADH is determined in the analyzer and under appropriate conditions is directly proportional to the plasma 3-hydroxybutyrate concentration:

Samples:
Sample volume:
Linearity:
Precision:

Plasma (heparin), serum
100 µl
5000 µmol/L (52 mg/dl)
Typical within-run CV = 3 - 4 % @ 480 µmol/L
 

Accuracy:

Serum/plasma vs SIGMA enzymatic spectrophotometric method
y (Analox) = 0.95x - 0.04 mmol/L, r = 0.985; n = 33
 

Reagent Stability:

Shelf-life 6 months unopened when stored at 0 - 5°C. Reconstituted
NAD reagent, 10 - 14 days if stored at 0 - 5°C.